临床肝胆病杂志2011，Vol.27Issue(2)：163-165,3.
乙型肝炎病毒对大鼠肝星状细胞活化的影响
The activation of rat hepatic stellate cells stimulated with hepatitis B virus in vitro
袁建国 1王新国 2崔霞 1刘凤华 1王凤华 1宋吉奎 1赵洪奎1
作者信息
- 1. 聊城市人民医院感染病科,山东,聊城,252000
- 2. 杭州市第六人民医院内二病区,杭州,510014
- 折叠
摘要
Abstract
Objective To observe the effects of HBV on rat hepatic stellate cells(HSC).Methods HSC were isolated by the method of Friedman and HBV was purified by concentrated sucrose gradient.HSC were stimulated with different concentrations of HBV for 24 hrs and the supernatant procollagen Ⅲ was found to be detected.Cells with increased procollagen Ⅲ secretion were selected to check for the intracellular C/EBPs, PPARγ, RAR expression to investigate if HBV had affected the HSC activation pathway.Results Secretion of procollagen Ⅲ by HBV stimulated rat HSC was concentration dependent.At the concentration of 3.0× 105 copies/ml, secretion of procollagen Ⅲ increased significantly.The expression of C/EBPs decreased significantly, while the RAR receptor expression was increased and PPARγ remained unchange.Conclusion HBV has a direct stimulus on HSC to promote the synthesis of procollagen Ⅲ, which may be associated with C/EBP decrease and RAR increase.关键词
肝炎病毒,乙型/肝细胞星形细胞分类
医药卫生引用本文复制引用
袁建国,王新国,崔霞,刘凤华,王凤华,宋吉奎,赵洪奎..乙型肝炎病毒对大鼠肝星状细胞活化的影响[J].临床肝胆病杂志,2011,27(2):163-165,3.
