中国农业科学2011，Vol.44Issue(3)：579-586,8.DOI:10.3864/j.ssn.0578-1752.2011.03.019
驴生长激素基因的克隆与分析
Cloning and Sequence Analysis of Growth Hormone Gene from Donkey
摘要
Abstract
[Objective] The objective of this study was to analyze the genetic differences of donkey growth hormone (GH)gene from its DNA, cDNA and deduced amino acid sequence in different species. [ Method ] The PCR primers were designed online according to result of gene homology comparison. Donkey GH gene DNA and cDNA sequence were cloned from liver tissue and blood by RT-PCR and PCR, and compared with GH gene sequences of different species by bioinformatics method. [Result] The donkey GH gene sequence was 1 928 bp, including the 706 bp cDNA sequence with the complete CDS. By comparison of DNA and cDNA sequences, it was found that the GH gene sequence included 5 exons and 4 introns, encoding 216 aa, including the signal protein of 26 aa and matured protein of 190 aa. Based on the analysis of the similarity of GH genes in different species on the level of cDNA, DNA and the deduced amino acid, there was the most homology to the horse. The GH gene of donkey was conservative in the process of evolution and its promotor was not specific TATA box, but CATA box. [ Conclusion ] DNA and cDNA sequences were obtained from liver tissue and blood, and the mutation of C→G DNA sequence in 1 267 may affect the growth and development of donkeys and horses. All researches made an essential foundation for GH regulation of gene expression, evolution,polymorphism analysis in the future.关键词
驴/生长激素基因/克隆/序列分析引用本文复制引用
朱文进,陈娟,关学敏,吴建华,耿立英,杨彩然,宋瑜,张艳英..驴生长激素基因的克隆与分析[J].中国农业科学,2011,44(3):579-586,8.基金项目
河北省自然科学基金项目(C2007000739)、河北科技师范学院博士启动基金项目(2006D006) （C2007000739）
