中国药房2011，Vol.22Issue(8)：758-759,2.
HPLC法测定复方黄连素片中盐酸小檗碱的溶出度
Dissolution Determination of Berberine Hydrochloride in Compound Berberine Tablets by HPLC
王红霞 1蒋立英2
作者信息
- 1. 江苏盐城市第一人民医院,盐城市,224001
- 2. 盐城卫生职业技术学院,盐城市,224001
- 折叠
摘要
Abstract
OBJECTIVE: To develop the method for the dissolution determination of berberine hydrochloride in Compound berberine tablets.METHODS: HPLC method was used.The determination was performed on Lichrospher ODS column with mobile phase consisted of 0.033 mol· L-1 acetonitrile-monobasic potassium phosphate solution (40:60) at detection wavelength of 265 nm.The injection volume was 10 μL.1 000 mL of 0.1 mol· L-1 artificial gastric juice was used as solvent.The rotation speed was 120 r· min-1, the sampling time was 45 min and the temperature was 37 ℃.RESULTS: The linear range of berberine hydrochloride was 0.021 5～0.172 0 ug (r=0.999 9) with an average recovery rate of 98.7％.CONCLUSION: The method is simple, accurate and reliable for dissolution determination of berberine hydrochloride in Compound berberine tablets.关键词
复方黄连素片/溶出度/高效液相色谱法分类
医药卫生引用本文复制引用
王红霞,蒋立英..HPLC法测定复方黄连素片中盐酸小檗碱的溶出度[J].中国药房,2011,22(8):758-759,2.
