中国农业科学2011，Vol.44Issue(3)：651-656,6.DOI:10.3864/j.ssn.0578-1752.2011.03.028
部分耐冬山茶栽培品种的AFLP分析
Study on the Genetic Diversity of Some Camellia japonica (Naidong) Cultivars Based on AFLP Markers
摘要
Abstract
[Objective] The genetic diversity of Camellia Japonica (Naidong) cultivar accessions was analyzed at molecular level, and the helpful information for cultivar classification was obtained. [Method] The genetic diversity of 16 cultivars and 2 mutation types of Camellia Japonica (Naidong) with 5 cultivars of C. japonica from south of China, 2 cultivars of C. retieulata was analyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoR Ⅰ/Mse 1 primer combinations. [Result] In the genomes of the 30 tested samples, a total of 1 062 spectral bands amplified from the 8 pairs of primer combinations were selected from AFLP analysis and 987 bands appeared to be polymorphic (92.9％). The data were used to calculate the Nei's genetic distance and to construct a dendrogram based on UPGMA cluster analysis. [Conclusion] It showed that the cultivars of Camellia japonica (Naidong) have a remote genetic relationship compared with the cultivars of Camellia from south of China, and cultivars of Camellia japonica (Naidong) can be divided into 6 groups, which indicated that Camellia japonica (Naidong) have rich genetic variability.关键词
耐冬山茶/AFLP标记/遗传多样性/亲缘关系引用本文复制引用
王奎玲,牟少华,刘庆超,唐启和,刘庆华..部分耐冬山茶栽培品种的AFLP分析[J].中国农业科学,2011,44(3):651-656,6.基金项目
山东省农业良种工程项目(鲁科农字[2006]90号)、青岛农业大学高层次人才启动基金项目 （鲁科农字[2006]90号）
