化学工程2011，Vol.39Issue(1)：81-84,4.
培养条件优化和菌种选育提高雷帕霉素发酵水平
Improvement of rapamycin fermentation with culture condition optimization and rational strain breeding
陈希杨 1朱湘成 1杨冬 1沈文和 1徐志南 1岑沛霖1
作者信息
- 1. 浙江大学,化学工程与生物工程学系,浙江,杭州,310027
- 折叠
摘要
Abstract
The optimal cultivation time of seed for rapamycin fermentation was investigated. It shows that the highest yield of fermentation with seed liquor can be achieved at 60 h. The effects of different medium components on rapamycin production were examined systematically, and one optimal fermentation medium was formulated. Under these optimal fermentation conditions, the yield of rapamycin is improved from 95 mg/L to 170 mg/L, achieving a big improvement by 79％ with the above optimization. The systematic research of rational screening techniques finds an applicable way to generate high-yield strains from original microorganisms. The highest yield reaches 420 mg/L, about 3 times higher than that of original strain.关键词
雷帕霉素/免疫抑制剂/培养条件优化/诱变育种Key words
rapamycin/ immunosuppressive agent/ culture optimization/ rational screening分类
化学化工引用本文复制引用
陈希杨,朱湘成,杨冬,沈文和,徐志南,岑沛霖..培养条件优化和菌种选育提高雷帕霉素发酵水平[J].化学工程,2011,39(1):81-84,4.
