生态与农村环境学报2011，Vol.27Issue(1)：84-87,4.
一种基于SDS溶液高效提取土壤残留Bt蛋白的方法
A Novel SDS-Buffer-Based Method for Extracting Bt Protein Residues From Soil.
摘要
Abstract
A novel SDS based method for extracting Bt protein residues from the soil was developed based on studies on extraction conditions including concentration of SDS in the solution and temperature and duration of incubation. Results show that when SDS was 2 g ·L- 1 in the solution at 50 ℃ for more than 4 hours it might extract effectively Bt protein residues from various types of soils, demonstrating better effect than the so-far known extraction methods, such as carbonate method, invertebrate gut fluid chemistry and PBST (phosphate buffer solution with Tween-20).关键词
SDS溶液/Bt蛋白/提取/土壤/碳酸盐法/人造蠕虫肠道蛋白提取液法/PBST法Key words
SDS buffer/ Bt protein/ extraction/ soil/ carbonate method/ invertebrate gut fluid chemistry/ phosphate buffer solution with Tween-20分类
资源环境引用本文复制引用
方志翔,孟军,贺昭和,刘标..一种基于SDS溶液高效提取土壤残留Bt蛋白的方法[J].生态与农村环境学报,2011,27(1):84-87,4.基金项目
转基因生物新品种培育科技重大专项(2008ZX08012-004,2008ZX08012-005,2008ZX08011-002) （2008ZX08012-004,2008ZX08012-005,2008ZX08011-002）
