环境工程学报2011，Vol.5Issue(1)：48-54,7.
一株芘的高效降解菌的选育及其降解性能研究
Breeding of a high-performance pyrene-degrading strain and its biodegradation performance
摘要
Abstract
A preponderant pyrene-degrading strain B-1 was isolated and purified from activated sludge collected from aeration basin of a coking plant to provide microorganism resource and scientific basis for bioremediation of the environment polluted by polycyclic aromatic hydrocarbons. Strain B-1 could grow and breed in liquid mineral salt medium with pyrene as sole carbon source and resist pyrene with concentration up to about 130 mg/L. Strain B-1 was initially identified as Bacillus by the results of morphological observation, physio-biochemical test and 16S rDNA sequence analysis. The incubation amount, initial pyrene concentration of liquid medium, pH and salinity are the factors to affect pyrene degradation efficiency. Under the conditions of incubation amount of 15％ (V/V), initial pyrene concentration of 20 ～100 mg/L and temperature of 30℃, strain B-1 could preserve high degradation activities of pyrene in the range of the pH 4 ～ 11 and the salinity less than 1％. With the initial pyrene concentration of 80 mg/L, incubation amount of 13％ V/V), pyrene degradation percentage by strain B1 was 92.4％ after 10 days.关键词
芽孢杆菌/16S rDNA/芘/生物降解Key words
Bacillus/ 16S rDNA/ pyrene/ biodegradation分类
环境科学引用本文复制引用
唐玉斌,马姗姗,王晓朝,陈芳艳..一株芘的高效降解菌的选育及其降解性能研究[J].环境工程学报,2011,5(1):48-54,7.基金项目
江苏省自然科学基金资助项目(BK2009726) （BK2009726）
江苏省高校自然科学基础研究资助项目(09KJD610002) （09KJD610002）
