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油污土中降解柴油细菌的分离鉴定及降解能力研究

曹冠楠 陈鸿汉 刘菲 郝春博

环境工程学报2011,Vol.5Issue(1):200-204,5.
环境工程学报2011,Vol.5Issue(1):200-204,5.

油污土中降解柴油细菌的分离鉴定及降解能力研究

Isolation and Identification of degrading bacteria in diesel contaminated soil and study on their degradation capability

曹冠楠 1陈鸿汉 1刘菲 1郝春博1

作者信息

  • 1. 中国地质大学(北京)水资源与环境工程北京市重点实验室,北京,100083
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摘要

Abstract

The biodegradation bacteria were enriched and isolated from the diesel contaminated soil. Two dominant strains were obtained and named as B-3, B-4. These strains were identified as Tetrathiobacter kashmirensis and Pseudomonas sp. through morphological observation and 16S rDNA sequence analysis, respectively.The degradation capabilities of strain B-3 and strain B-4 were studied in this paper. The strains B-3 and B-4 were taken in the culture medium, in which diesel concentration was 3.78 g/L. The degradation rate of them was 60.98% and 37. 18% , respectively. And the results of morphological observation and growth curve measurement to strain B-3 showed that the degradation capability is obviously higher than B-4. To our knowledge, this is also the newly established species about biodegrading diesel contaminated soil using strain B-3.

关键词

柴油污染/生物降解/菌株鉴定/降解率

Key words

diesel oil contamination/ biodegration/ identification of strains/ degradation rate

分类

资源环境

引用本文复制引用

曹冠楠,陈鸿汉,刘菲,郝春博..油污土中降解柴油细菌的分离鉴定及降解能力研究[J].环境工程学报,2011,5(1):200-204,5.

基金项目

国际科技合作项目(2006DFA21180) (2006DFA21180)

环境工程学报

OA北大核心CSCDCSTPCD

1673-9108

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