上海海洋大学学报2011，Vol.20Issue(1)：89-95,7.
感染鳗弧菌对花鲈非特异性免疫功能的影响
The impact of Vibrio anguillarum challenging on non-specific immune responses of sea perch Lateolabrax japonicus
摘要
Abstract
Vibrio anguillarum is a common form of bacterial pathogens for marine fish. In this paper, sea perch (Lateolabrax japonicus) was challenged with an intraperitoneal injection at different concentrations of V.anguillarum: namely treatments A: 1 × 106 pfu/mL, B: 1 × 107 pfu/mL, C: 1 × 108 pfu/mL and the innate immunity indicators were investigated. Blood samples were taken at different time, and the effects of challenging of V. anguillarum on the immunity responses were studied by measuring the respiratory burst,alkaline phosphatase (ALP), acid phosphatase (ACP), phenoloxidase (PO) and superoxide dismutase (SOD) activities. The results showed that: 1 ) Compared with the control group, the respiratory burst activity of treatment B and C decreased significantly (P ＜0.01 ) at 12h and 36h of injection. 2) Compared with the control group, the PO activity of treatment A, B and C at 12 h, 36 h ,60 h, 84 h and 108 h after injection decreased significantly (P ＜ 0.01 ). 3 ) There was no significant difference between treatments and control groups for ALP activity except for at 60 h. 4) Compared with the control group, the ACP activity of treatment A, B and C at 60 h, 84 h and 108 h after injection increased significantly ( P ＜ 0.05 ). 5 ) There was no significant difference between treatments and control groups for SOD activity at 12 h, 36 h, 60 h, 84 h and 108 h(P ＞0.05). Moreover, in the semi-lethal experiment, V. anguillarum at the concentration of 1 × 106 pfu/mL, 1 × 107 pfu/mL and 1 × 108 pfu/mL 50％, of death in experimental group occurred on 12 d, 11 d and 8d respectively, while no death for control group. During infection, experimental group has different symptoms: caudal fin ulceration, gill pale, whitish cornea, the mouth's ulceration, the inside has yellow viscous ascites. The higher the concentration infection with V. anguillarum, the earlier was for 50％ of the death. These results indicated that V. anguillarum concentration and the mortality rate are positively correlated.关键词
花鲈/鳗弧菌/非特异性免疫/半致死时间分类
农业科技引用本文复制引用
郭伟荣,刘利平,张宗锋,林升..感染鳗弧菌对花鲈非特异性免疫功能的影响[J].上海海洋大学学报,2011,20(1):89-95,7.基金项目
上海市教育委员会科研(创新)项目(09YZ277) （创新）
上海市青年科技启明星项目(08QA1405900) （08QA1405900）
上海市重点学科建设项目(Y1101) （Y1101）
美国国际发展署(USAID) （USAID）
AquaFish CRSP项目(3001325371) （3001325371）
