上海海洋大学学报2011，Vol.20Issue(1)：15-21,7.
草鱼胰岛素样生长因子结合蛋白IGFBP-1基因的全长cDNA克隆及表达
cDNA cloning and expression of insulin-like growth factor binding protein gene IGFBP-1 in Ctenopharyngodon idellus
摘要
Abstract
In this study, the complete cDNA of a insulin-like growth factor binding gene IGFBP-1 was cloned by rapid amplification of cDNA ends (RACE) in grass carp, Ctenopharyngodon idellus. And mRNA transcripts during embryogenesis and in adult tissues were studied by RT-PCR. The results showed that: ( 1 )The complete cDNA of C. idellus IGFBP-1 is 1 135 bp and its ORF includes 789 bp which coding 262 aminoacid residue. The deduced amino acid analysis showed that the sequence similarities between C. idellus and Cyprinus carpio , Danio rerio , Ictalurus punctatus, Salmo salar , Oncorhynchus mykiss , Seriola quinqueradiata,Mus musculus and Homo sapiens are 94％, 93％, 69％, 60％, 58％, 56％, 40％ and 38％, respectively.Its functional N-terminal and C-terminal domains, which are involved in binding with IGF ligand, are very conservative. (2) RT-PCR results demonstrated that IGFBP-1 gene had a iow expression in embryos at different stages in C. idellus. Weak mRNA transcripts can be detected after 12 hpf. Tissue distribution results showed that IGFBP-1 mRNA was expressed in liver, kidney, intestines and heart. In terms of its crucial function of IGFBP-1 in IGF signal pathway and as a hypoxia-inducible gene, our results provide data for further functional investigations of IGFBP-1 gene in grass carp.关键词
草鱼/胰岛素样生长因子结合蛋白1/全长cDNA/RTPCR分类
农业科技引用本文复制引用
陶洋,邹曙明..草鱼胰岛素样生长因子结合蛋白IGFBP-1基因的全长cDNA克隆及表达[J].上海海洋大学学报,2011,20(1):15-21,7.基金项目
国家"八六三"高技术研究发展计划项目(2009AA10Z105) （2009AA10Z105）
公益性行业(农业)科研专项(200903045) （农业）
上海市教委曙光计划(08SG50) （08SG50）
