兽类学报2011，Vol.31Issue(1)：108-112,5.
紫色色杆菌感染小熊猫引起肺炎的临床调查
Clinical investigation of pneumonia in the red panda(Ailurus fulgens)caused by the non-pigmented strain of Chromobacterium violaceum
摘要
Abstract
This clinical investigation reports several cases of pneumonia in the red panda ( Ailurus fulgens ) which occurred at Fuzhou Giant Panda Research Center in July, 2008. Among 7 infected animals, 3 red pandas died within 3 days after showing symptoms of high fever and severe respiratory disease. Necropsy results showed a white foamy discharge in the trachea; suppurative necrosis and massive congestion in the lung; turbid pleural and pericardial effusions; a liver with multiple atrophic foci and focal congestive necrosis. Through bacterial isolation and identification, the pathogen was confirmed to be non-pigmented Chromobacterium violaceum. Based on GenBank database of 16S RNA gene sequences for Chromobacterium violaceum, primer pairs ( 5' GAG CAA ACA GGA TTA GAT ACC 3 '; 5' TTA CGG TrA CCT TGT TAC GAC 3' )were designed to amplify a 739 bp gene fragment by PCR. The nucleotide sequences obtained subsequently were compared to seven strains of Chromobacterium violaceum from the GenBank database and found to be 98.8％ identical to strains CV09c and ESBV4400 as well as 98.2％, 98.0％, 94.9％, 93.1％ and 92.8％ identical to strains AY117554, EAV2,AJ871127, LMG3953 and JS1, respectively. Intraperitoneal inoculation of 5 mice with the isolated pathogen culture resulted in the death of all mice within 2 - 3 days, a reflection of the virulence of this isolate. Several measures were implemented to control the spread of infection including disinfecting of the grounds, housing environment, and water supply. We further tested the clinical isolate for antibiotic susceptibility and based on these findings, the 4 remaining asymptomatic pandas were all treated twice daily intramuscularly with Cefoperazone sodium for two days in combination with oral dosing of Sulfamethoxazole twice a day for four days, and no new case was seen after the treatments. This investigation indicates that the rapid onset of infection and high fatality rate of Chromobacterium violaceum in the red panda should be noted and we conclude that trauma or injury to these animals in combination with pathogen contaminated surroundings and water supplies are the main reasons for onset of the infection.关键词
小熊猫/紫色色杆菌/肺炎/临床调查分类
农业科技引用本文复制引用
修云芳,邵良平,李碧春,徐素慧,吴尚明,王隆伯,王德春,周伦江,陈玉村..紫色色杆菌感染小熊猫引起肺炎的临床调查[J].兽类学报,2011,31(1):108-112,5.基金项目
福建省生物技术科技项目(2000Z156) （2000Z156）
