兽类学报2011，Vol.31Issue(1)：103-107,5.
梅花鹿S100A4基因的克隆及融合蛋白的表达
Cloning and fusion protein expression of the S100A4 gene in sika deer
摘要
Abstract
In order to better understand the function of S100 calcium binding protein A4 in antler development of sika deer ( Cervus nippon), we cloned S100A4 genes from total RNA of cultured antlerogenic periosteum cells using reverse transcription polymerase chain reaction ( RT-PCR), S100A4 gene sequences were compared with closely related animal species in NCBI. Full lengths of S100A4 genes were inserted into a vector plasmid pLEGFP-C1 ( retroviral express vector). The recombinant plasmid pLEGFP-S100 and pVSV-G (envelope vector) were co-transfected into GP2 -293 cells (packaging cell line ) using lipofectimin 2000, and the resultant viral supernatants were collected. The cultured pedicle periosteum cells were then infected with virus in the supernatants. Results showed that S100A4 gene was a relatively conserved gene, and had about 90％ homology with several species. Recombinant retroviral vector pLEGFP-S100 could effectively deliver a gene into target cell line, and express a fusion GFP ( green fluorescent protein ) protein.关键词
S100A4基因/肿瘤/鹿茸/逆转录病毒载体/融合蛋白分类
生物学引用本文复制引用
王大涛,赵海平,褚文辉,杨福合,邢秀梅,王桂武,李春义..梅花鹿S100A4基因的克隆及融合蛋白的表达[J].兽类学报,2011,31(1):103-107,5.基金项目
国家自然科学基金资助项目(309771664) （309771664）
