中国兽医科学2011，Vol.41Issue(1)：70-74,5.
仔猪胰腺干细胞的多向分化潜能
Multiple differentiation potentialities of pancreatic stem cells in neonatal piglets
摘要
Abstract
The pancreatic stem cells, isolated from 1-day-old neonatal piglets, would be proved that they had the multipotent potentiality of stem cells by the inducing tests toward the function cells of pancreatic and triploblastic. The RPMI 1640 medium supplemented nicotinamide and hepatic growth factor was the differentiating medium toward endocrine β-cell of islets. The differentiating medium toward myocardium contained 5-Azacitidine. The RPMI 1640 medium associated with dexamethasone,β-glycerophosphate and ascorbic acid differentiated the cells toward the osteoblast lineage. To differentiate toward neural cells, the medium was supplemented different concentration β-mercaptoethanol. The results demonstrated that the isolated pancreatic stem cells were induced the differentiation toward endocrine β-cell,and stained dithizonepositive. The quantity of the secreted insulin varied with the different concentration of glucose. The pancreatic stem cells of differentiating toward myocardium expressed a-actin myocardial protein, and presented positive reaction of periodic acid Schiff. The isolated cells toward the osteoblast lineage were stained typical bright red with Alizarin Red and alkaline phosphatase. The induced cells expressed many markers of neural cells,which included GAFP, BMP and NF-200. In conclusion,the isolated stem cells from pancreas have the multipotent potentiality differentiating toward triploblastic function-cells in neonatal piglets.关键词
胰腺干细胞/仔猪/多向分化潜能/诱导分类
生物科学引用本文复制引用
张慧茹,冯若鹏,庞全海,刘珍,窦忠英..仔猪胰腺干细胞的多向分化潜能[J].中国兽医科学,2011,41(1):70-74,5.基金项目
国家高技术研究发展计划(863)项目(2005AA219050) （863）
国家自然科学基金资助项目(30671067) （30671067）
河南工业大学人才引进项目(150214) （150214）
