中国兽医科学2011，Vol.41Issue(1)：5-8,4.
狂犬病病毒SRV-9株对小鼠脑神经原代细胞的感染及毒力观察
Observation on infection and virulence of rabies virus SRV-9 strain on murine primary neuron cells
摘要
Abstract
To establish a method used for infecting primary neuron cells by rabies virus in vitro and made some observations on toxicity and adaptability after infection, primary neuron cells were isolated and cultured from newborn mice which were infected with rabies virus SRV-9 strain later,and the virus was detected and the influence of the virus on the primary neuron cells was analyzed by the immune fluorescence technology and the immunocytochemistry tests. In result, the murine primary neuron cells were successfully infected by rabies virus and the specific fluorescence was detected. These results showed that the method for preparation of primary neuron cells suitable for culturing rabies virus was successfully developed and its toxicity change can be observed when murine primary neuron cells adapt to the infection of the virus.关键词
狂犬病病毒/神经细胞/原代培养/免疫荧光分类
农业科技引用本文复制引用
胡乐鹏,王利民,夏志平,徐翠平,刘存霞,李志萍,李勇,刘雯,袁子国,张宁..狂犬病病毒SRV-9株对小鼠脑神经原代细胞的感染及毒力观察[J].中国兽医科学,2011,41(1):5-8,4.基金项目
农业公益性行业项目(200803014) （200803014）
