中国水产科学2011，Vol.18Issue(1)：110-118,9.DOI:10.3724/SP.J.1118.2011.00110
微胶囊蛋氨酸或晶体蛋氨酸对军曹鱼幼鱼相关酶活性的影响
Effect of supplementation microcapsule or crystalline methionine in diets on related enzyme activity of cobia(Rachycentron canadum)
摘要
Abstract
The present study was conducted to compare the effect of microcapsule methionine or crystalline methionine in low-fishmeal diets on protein metabolism and digestive enzyme activities in cobia (Rachycentron canadum). Seven iso-nitrogen and iso-lipid diets, including fishmeal (positive control), low-fishmeal (negative control) and five types of methionine supplementation of crystalline L-methionine(MET), hydroxyl-methionine calcium(MHA), celloiose-acetate-phthalate coated methionine(CAP), resin coated methionine (RES) and tripalmitin-polyvinyl alcohol coated methionine(TPA), respectively were prepared to investigate utilization of coated and crystalline methionine in intestine of juvenile cobia. Each treatment was randomly assigned to triplicate groups of 20 fish with initial weight of (5.40±0.07) g per aquarium. Fish were maintained in flow- through aquaria for eight weeks at water temperature ranged from 29＜℃ to 31 ℃. The results showed that trypsin activities of intestine of fish fed microcapsule methionine were significantly higher than those of fish fed crystalline methionine and the positive control diet(P＜0.05). Compared to positive and negative groups, fish fed the diets with MET and TPA had significant difference in liver GOT activities (P＜0.05). After feeding 0.5 h, Na+, K+-ATP activities in intestine of fish fed MET were significantly higher than those of fish in other groups (P＜0.05). After feeding 3 h,Na+, K+-ATP activities of fish fed RES and positive control diet increased and was higher than those of fish fed CAP and MHA, and those activities of fish fed positive control diets and microcapsule methionine were significantly higher than those offish fed MHA and MET after feeding 8 h (P＜0.05). It could be concluded that cobia fed low fishmeal diet with crystalline methionine could improve their metabolism of amino acid and synthesis of protein. Microcapsule could contribute to controll methionine release in intestine and enhance the activities of intestinal protease. [Journal of Fishery Sciences of China, 2011, 18(1): 110-118]关键词
军曹鱼/蛋氨酸/微胶囊/消化酶/蛋白质代谢酶分类
农业科技引用本文复制引用
迟淑艳,谭北平,董晓慧,杨奇慧,刘泓宇,许悦娜,黄红岩..微胶囊蛋氨酸或晶体蛋氨酸对军曹鱼幼鱼相关酶活性的影响[J].中国水产科学,2011,18(1):110-118,9.基金项目
广东省教育厅引进人才专项资金(0909030) （0909030）
广东海洋大学自然科学基金项目资助(0612188) （0612188）
广东海洋大学自然科学研究项目(0812173). （0812173）
