中国兽医科学2011，Vol.41Issue(1)：1-4,4.
山羊痘病毒感染绵羊的诊断及其p32基因的分子分析
Diagnosis of an outbreak of sheep pox associated with goatpox virus and molecular analysis of p32 gene of the virus
摘要
Abstract
The GPV/GSgulang/09 strain of goatpox virus(GPV) was isolated from lung tissues of Small-tailed Han sheep suffered from pox disease by inoculating into Vero cells. PCR-RFLP and sequence analysis of p32 gene were conducted for classifying this virus. Two bands,which are specific to goatpox virus,were observed after the p32 gene was amplified from genomic DNA of GPV/GSgulang/09, and then digested with the endonuclease of Hinf Ⅰ . Three nucleotide bases for encoding residue of aspartic acid which are located at 166 to 168 positions of p32 gene of sheeppox virus (SPV) were absent in that of GPV/GSgulang/09. Comparing the sequence of the p32 gene amplified from GPV/GSgulang/09 strain with those available in GenBank,the results showed that GPV/GSgulang/09 shared the homology of 98. 1％ to 98.3％ with SPV and 99.2％ to 99. 9％ with GPV, respectively. In the phylogenetic analysis, GPV/GSgulang/09 was more closely related to GPV strains and clustered into GPV sub-branch of phylogenetic tree constructed based on p32 gene. These results showed that the outbreak of sheep pox associated with GPV occurred for the first time in China.关键词
山羊痘病毒/绵羊/感染/p32基因分类
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颜新敏,吴国华,李健,朱海霞,赵志荀,崔力凡,朱彩珠,张强..山羊痘病毒感染绵羊的诊断及其p32基因的分子分析[J].中国兽医科学,2011,41(1):1-4,4.基金项目
甘肃省重大科技专项(092NKDA032) （092NKDA032）
国家科技支撑计划项目(2006BAD06A11,2006BAD06A17) （2006BAD06A11,2006BAD06A17）
