中国水产科学2011，Vol.18Issue(1)：29-37,9.DOI:10.3724/SP.J.1118.2011.00029
基于16S rDNA序列的中国沿海短蛸种群遗传结构
Population genetic structure of Octopus ocellatus in coastal waters of China based on 16S rDNA sequence
摘要
Abstract
Genetic structure of populations is greatly influenced by the dispersal ability of marine organisms. It seems intuitive that limited dispersal capability should result in greater genetic differentiation. Octopus ocellatus is a typical cephalopod species which shows limited dispersal potential. In this study, the effect of dispersal capability on population genetic structure was tested on seven populations of O. ocellatus in coastal waters of China,by applying mitochondrial gene sequencing technology. A 485 bp segment of the 16S rDNA gene was sequenced and analyzed in 100 specimens. Results showed that strong genetic structure existed in seven O. ocellatus populations. Two distinct clades with 14 fixed nucleotide base variation in the 16S rDNA gene region were detected: one was composed of four populations from northern coastal waters, including Dalian, Yantai, Qingdao, Lianyungang populations, the other consisted of three populations from southern coastal waters including Shanghai, Zhoushan,Guangzhou populations. The FST and Nm between the two clade were high, which amounted to 0.878 and 0.069,respectively. AMOVA test, which showed 12.24％ of the genetic variation lied within populations and 87.76％ lies between populations, further suggested there was genetic differentiation between the two lineages. The net genetic distance between the two lineage was 0.019, suggesting a moderate and intraspecific level of differentiation. The two lineages may diverge in late Pleistocene, suggested by molecular clock analysis. The divergence might be resulted from low dispersal ability in O. ocellatus at both larva and adult stages. Pattern of isolation by distance was observed in this species, further verifying this point of view. The frequent osilation of sea level during the late Pleistocene and the fresh water outflow from Changjiang River might also play an important role in the formation of genetic structure in O. ocellatus populations along China coasts. These findings will have important implications for better exploitation and protection of O. ocellatus resources in China in the near future.[Journal of Fishery Sciences of China, 2011, 18(1): 29-37]关键词
短蛸/16S rDNA/种群遗传结构分类
农业科技引用本文复制引用
吕振明,李焕,吴常文,樊甄姣,张建设..基于16S rDNA序列的中国沿海短蛸种群遗传结构[J].中国水产科学,2011,18(1):29-37,9.基金项目
国家科技支撑计划项目(2007BAD43B00) （2007BAD43B00）
国家863计划项目(2010AA10A404). （2010AA10A404）
