中国兽医科学2011，Vol.41Issue(1)：14-18,5.
适合猪圆环病毒2型敏感复制的PK15细胞株的克隆与鉴定
Cloning and identification of PK15 cells for the sensitive replication of porcine circovirus 2
摘要
Abstract
To improve the titers of porcine circovirus 2(PCV2),PK15 cells were cloned using the limiting dilution method and the susceptible cells to PCV2 were selected by indirect immunofluorescence assay.In result,one clone,PK15-B1, could reliably produce PCV2 with high titers was selected from heterogeneous PK15 parental cells.The viral titer could be as high as 107.0TCID50/mL after the PK15-B1 cells inoculated with 100 TCID50 for 3 days.The levels of the viral titer and growth speed in PK15-B1 cells were significantly higher than those in PK15 parental cells.Moreover, the clone cells did not contaminated with porcine circovirus 1, bovine viral diarrhea virus, classical swine fever virus, encephalomyocarditis virus or mycoplasma.The results indicated that the PK15-B1 cell clone is reliably permissive to PCV2 replication and could be used to study the biological characterization of PCV2 and replication of PCV2 in vitro.关键词
猪圆环病毒2型/PK15细胞/克隆分类
农业科技引用本文复制引用
陈念劬,王先炜,李玉峰,姜平..适合猪圆环病毒2型敏感复制的PK15细胞株的克隆与鉴定[J].中国兽医科学,2011,41(1):14-18,5.基金项目
农业公益性行业专项(200803020-5,201003060) （200803020-5,201003060）
江苏省教育厅产业发展项目(JH09-1) （JH09-1）
教育部博士点基金项目(20090097120025) （20090097120025）
