安徽医科大学学报2011，Vol.46Issue(3)：228-230,3.
SOCS1对α干扰素调节乙肝病毒复制作用的影响
Effect of SOCS1 on the antiviral replication of interferon-α
摘要
Abstract
Objective To investigate the relationship between SOCS1 and the antiviral replication of IFN-α. Methods HepG2.2. 15 cells were divided into three groups,blank group( HepG2. 2. 15 cells without 1 000 IU/ml IFNα treatment), control group ( transfected with pcDNA3. 1 plasmid ), experimental group ( transfected with pcDNA3. 1SOCS1 plasmid ). After transfection, real-time PCR, Western blot and chemiluminescence assay were employed to detect the expression of SOCS1 protein, HBV DNA, HBsAg and HBeAg. Results IFN-ot could inhibit the HepG2. 2. 15 cell HBV replication. SOCSl protein expressed in HepG2. 2. 15 cells transfected with pcDNA3. lSOCS1 (wild-type)plasmid. After treated with 1 000 IU/ml IFN-α, we found that compared with the control group,the expression of HBeAg, HBsAg and HBV DNA in experimental group statistically significant increased, showing significant difference between the two groups( P ＜ 0. 05 ). Conclusion SOCS1 can affect the process of IFN-o inhibiting HepG2. 2. 15 cell HBV replication in vitro which provide the way for further studying the regulatory mechanism of HBV resist to IFN-α.关键词
肝炎病毒,乙型/干扰素α/细胞因子信号转导蛋白抑制因子Key words
hepatitis B virus /interferon-alpha/ suppressor of cytokine signaling proteins分类
医药卫生引用本文复制引用
程中乐,管世鹤,杨凯,潘颖..SOCS1对α干扰素调节乙肝病毒复制作用的影响[J].安徽医科大学学报,2011,46(3):228-230,3.基金项目
国家自然科学基金(编号:30600522) （编号:30600522）
安徽省教育厅资助项目(编号:2010C057) （编号:2010C057）
