分析测试学报2011，Vol.30Issue(2)：222-224,228,4.DOI:10.3969/j.issn.1004-4957.2011.02.021
高效液相色谱法同时测定香兰素与邻位香兰素
Simultaneous Determination of Vanillin and o-Vanillin by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatographic method was developed for the simultaneous separation and determination of vanillin and o-vanillin. Influences of mobile phase composition and column temperature on the separation were investigated. Under the condition of 5％ acetic acid -acetonitrile (60: 40, by volume) as mobile phase at a flow rate of 1.0 mL/min and 25 ℃, the vanillin and o-vanillin were separated in less than 5 min. The calibration curves of vanillin and o-vanillin showed good linearities in the range of 10 - 240 mg/L with both correlation coefficients of 0. 999 7.The limits of detection ( S/N = 3 ) for vanillin and o-vanillin were 0. 06 mg/L and 0. 02 mg/L, respectively. The average recoveries at three spiked levels were in the range of 99％ - 102％ with relative standard deviations of 0. 2％ - 0. 6％. The method was successfully used for the determination of vanillin and o-vanillin in real samples with satisfactory results.关键词
高效液相色谱法/香兰素/邻位香兰素/同分异构体Key words
high performance liquid chromatography/ vanillin/ o-vanillin/ isomers分类
化学化工引用本文复制引用
金海涛,马健瑜,王晓珣,陈星,任丽艳,关瑾,李云..高效液相色谱法同时测定香兰素与邻位香兰素[J].分析测试学报,2011,30(2):222-224,228,4.基金项目
辽宁省自然基金资助项目(20090655) （20090655）
辽宁省教育厅2008年度科研计划项目(2008S184) （2008S184）
