广西医学2011，Vol.33Issue(1)：5-7,3.
表没食子儿茶素没食子酸酯体外代谢的方法研究
Metabolism Method of(-)-epigallocatechin-3-O-gallate in Vitro
摘要
Abstract
Objective To study the metabolism method of( - )-epigallocatechin-3-O-gallate( EGCG )in vitro and its metabolites measured by high performance liquid chromatography( HPLC ). Methods EGCG was metabolized in rat liver homogenate in vitro with S-adenosylmethionine( SAM ), Mg2+ under appropriate temperature and pH values, and then the content of EGCG and its metaboltes were detected by HPLC. Results EGCG in vitro was metabolized efficiently. EGCG and its metabolites were analyzed by HPLC. Conclusion The metabolism method of EGCG in vitro is simple, convenient and accurate. It can be used to study the metabolism of EGCG effectively in vitro.关键词
表没食子儿茶素没食子酸酯/体外代谢/高效液相色谱法Key words
Epigallocatechin-3-O-gallate/Metabolism in vitro/High performance liquid chromatography分类
医药卫生引用本文复制引用
廖作庄,梁钢,欧冰凝..表没食子儿茶素没食子酸酯体外代谢的方法研究[J].广西医学,2011,33(1):5-7,3.基金项目
国家自然科学基金项目(30760310) （30760310）
广西科学基金(桂科自0728113) （桂科自0728113）
广西科技厅广西中药质量标准研究重点实验室开放课题基金项目(桂中开0801) （桂中开0801）
