解剖学杂志2011，Vol.34Issue(1)：52-54,3.
黄芩茎叶总黄酮预处理对脑缺血再灌注皮质神经元热休克蛋白70及超微结构的保护作用
Protective effect of scutellaria baicalensis stem-leaf total flavonoid on expression of heat shock protein 70 and ultrastructure in cortex neurons following cerebral ischemia reperfusion
摘要
Abstract
Objective: To investigate the effect of scutellaria baicalensis stem-leaf total flavonoid (SSTF) on the expression of heat shock protein 70 (HSP70) and ultrastructure in the cortex neuron induced by focal cerebral ischemia reperfusion (IR) in rats.Methods: The model of focal cerebral ischemia reperfusion was built up on rats using the suture embolization.The expression of HSP70 and ultrastructure of the cerebral cortex were observed by immunohistochemical techniques and electron microscopy.Results: A few positive cells were expressed (0.017±0.011) and its ultrastructure looked normal in the sham operated group; in the model group, the positive area rate of HSP70 increased (0.038 ±0.010), many necrosis were obeserved, part of the membrane was dissolved, rnitochondria was swelling with endoplasmic reticulum dilatation, nuclear membrane was incomplete, and chromatin showed massive agglutination.Compared to the model group, the positive area rate of HSP70 increased sharply (0.069±0.010), the shape of cells was relatively regular, apoptosis was rare, mitochondria swelling decreased, endoplasmic reticulum and nuclear membrane tended to normal and complete, and chromatin relatively showed neurons in the SSTF interferred group.Conclusion: SSTF can protect the cerebral cortex from IR injury by upregulating the expression of HSP70 and improving the ultrastructure of the neurons.关键词
脑缺血再灌注/热休克蛋白70/超微结构/黄芩茎叶总黄酮/大鼠Key words
cerebral ischemia reperfusion/ heat shock protein 70/ ultrastrncture/ scutellaria baicalensis stem-leaf total flavonoid/ rat引用本文复制引用
郑小影,孔祥玉,赵淑敏..黄芩茎叶总黄酮预处理对脑缺血再灌注皮质神经元热休克蛋白70及超微结构的保护作用[J].解剖学杂志,2011,34(1):52-54,3.基金项目
河北省科技厅,河北省教育厅资助课题(07276101D-46,2005227) （07276101D-46,2005227）
