河南农业科学2011，Vol.40Issue(1)：48-53,6.
我国亚热带地区快生型大豆根瘤菌遗传多样性研究
Genetic Diversity of Fast-growing Rhizobia Associated with Glycine max in Subtropical Regions of China
摘要
Abstract
Genetic diversity of 31 isolates from effective nodules of soybean in different geographical regions of China was studied using 16S rDNA gene RELP patterns, 16S rDNA gene sequencing, 16S— 23S rDN A IGS region RE LP patterns and 16S — 23S rDN A IGS sequencing assays. The isolates were clustered into one genospecies with Sinorhizobium fredii USDA205 on the basis of their 16S rDNA gene PCR - RFLP patterns. 16S rDNA gene sequence of strains indicated that the isolates were very closely related (identities higher than 99.5％) to Sinorhizobium fredii USDA205. The analysis of the 16S—23S intergenic spacer (IGS) divided the isolates into 10 genotypes and four groups. The 16S—23S rDNA IGS sequencing assays divided the isolates into two groups. Group Ⅰ was clustered with Sinorhizobium fredii USDA205. Group Ⅱ was less similarity to Sinorhizobium fredii USDA205 than Sinorhizobium xinjiangense. The isolates from the subtropical regions of China had higher genetic similarity.关键词
大豆根瘤菌/多样性/16S rDNA/基因间区Key words
Soybean rhizoia/ Diversity/ 16S rDNA/ IGS分类
农业科技引用本文复制引用
杨成运,周俊初,杨江科,段广才..我国亚热带地区快生型大豆根瘤菌遗传多样性研究[J].河南农业科学,2011,40(1):48-53,6.基金项目
科技部微生物资源课题(2005DKA21208-6) （2005DKA21208-6）
国家高技术研究发展计划(2007AA5Z417) （2007AA5Z417）
华中农业大学农业微生物学国家重点实验室开放基金 （）
