解剖学杂志2011，Vol.34Issue(1)：45-47,84,封面,5.
Notch-1对嗅成鞘细胞脑源性神经营养因子表达的影响
Effect of Notch-1 on expression of BDNF in olfactory ensheathing cells
摘要
Abstract
Objective: To observe the effect of Notch-1 on the expression of BDNF in olfactory ensheathing cells (OECs), so as to provide experimental basis on enhancing the recovery ability of OECs.Methods: Active Notch plasmid (NotchICV), inactive Notch plasmid (NotchVK) and empty plasmid were transfected into OECs.Immunofluorescence, RT-PCR and Westem blot were used to detect the expression changes of BDNF and its mRNA.Results: The expression of BDNF and its mRNA in the NotchICV group was increased compared with those in the NotchVK group and control group; however, there was no difference between the NotchVK group and control group.Conclusion: After Notch-1 being transfected into OECs, the expression of BDNF increases.It suggests that Notch-1 could improve the synthesis and secret of BDNF.关键词
Notch/嗅成鞘细胞/脑源性神经营养因子Key words
Notch/ olfactory ensheathing cells/ brain-derived neurotrophic factor引用本文复制引用
卓飞,孙善全,余维华,刘辉,邱国平,杨美..Notch-1对嗅成鞘细胞脑源性神经营养因子表达的影响[J].解剖学杂志,2011,34(1):45-47,84,封面,5.基金项目
国家自然科学基金(30670743) （30670743）
重庆市科委自然科学基金(2008BB5224) （2008BB5224）
