解剖学杂志2011，Vol.34Issue(1)：41-44,4.
白细胞介素-1β和胶质源性神经营养因子体外诱导鼠胚中脑神经干细胞向酪氨酸羟化酶阳性神经元分化
IL-1β and GDNF induce mouse embryonic mesencephalic neural stem cells to differentiate into tyrosine hydroxylase positive neurons in vitro
罗特坚 1丁继固 2李光3
作者信息
- 1. 邵阳医学高等专科学校解剖学教研室,邵阳422000
- 2. 咸宁学院医学院解剖学教研室,咸宁437100
- 3. 武汉大学中南医院重症医学科,武汉430071
- 折叠
摘要
Abstract
Objective: To explore mouse embryonic neural stem cells to dopaminergic neurons division induced by IL-1β and GDNF, and provide the experimental basis for the mesencephalic neural stem cells (M-NSCs) transplantation for treatment of Parkinson's disease. Methods: M-NSCs were cultured and proliferated, and then IL-1β and GDNF were used to induce differentiation in the condition of fetal bovine serum. Immunocytochemical techniques were used to detect the TH-ir cells from M-NSCs differentiation, and Flow cytometry was used to detect the percentage of TH-ir in all the cells. Results: The proportions of M-NSCs differentiation into TH positive cells were as follows: 13. 53％ in the GDNF group, 9. 66％ in the IL-1β group, 16.22％ in the GDNF+IL-1β group. The data of each group were much higher than those in the blank control group (3. 46 ％). The proportions of NSCs differentiation into TH positive cells showed significant difference among experimental groups. Conclusion: IL-1β and GDNF can significantly increase the differentiation of dopaminergic neurons.关键词
白细胞介素-1β/胶质源性神经营养因子/酪氨酸羟化酶/中脑神经干细胞/多巴胺能神经元Key words
interleukin-lβ/ glial cell line-derived neurotrophic factor/ tyrosine hydroxylase/ mesencephalic neural stem cells/ dopaminergic neurons引用本文复制引用
罗特坚,丁继固,李光..白细胞介素-1β和胶质源性神经营养因子体外诱导鼠胚中脑神经干细胞向酪氨酸羟化酶阳性神经元分化[J].解剖学杂志,2011,34(1):41-44,4.
