烟草科技Issue(1)：36-42,7.
巴豆醛诱导人支气管上皮细胞凋亡研究
Caspase-mediated Apoptosis of Human Bronchial Epithelial Cells Induced by Crotonaldehyde
刘兴余 1杨陟华 2潘秀颉 3朱茂祥 3谢剑平3
作者信息
- 1. 中国科学院大连化学物理研究所,辽宁省大连市中山路457号,116023
- 2. 上海烟草(集团)公司技术中心北京工作站,北京市通州区万盛南街99号,101121
- 3. 军事医学科学院放射与辐射医学研究所,北京市太平路27号,100850
- 折叠
摘要
Abstract
In order to assess the health risk of cigarette smoke, crotonaldehyde-induced cell death and its mechanism in BEAS -2B cells were investigated.Cell survival rate, intracellular ATP level, apoptosis rate, cytochrome c released from mitochondria to cytoplasm, and caspase - 9 and caspase - 3/7 activities were determined by MTT, chemiluminescence, flow cytometry, flow cytometry combined with immunochemistry, and chemiluminescence methods, respectively.The results showed that crotonaldehyde induced cell apoptosis, and turned into necrosis along with the increase of crotonaldehyde dosage.Crotonaldehyde caused that intracellular ATP level declined rapidly, mitochondria membrane potential decreased, cytochrome c released from mitochondria to cytoplasm, and caspase - 9 and caspase - 3/7 were activated.It was concluded that crotonaldehyde induced and caspase mediated cell apoptosis.关键词
巴豆醛/凋亡/caspase分类
轻工纺织引用本文复制引用
刘兴余,杨陟华,潘秀颉,朱茂祥,谢剑平..巴豆醛诱导人支气管上皮细胞凋亡研究[J].烟草科技,2011,(1):36-42,7.
