分析测试学报2011，Vol.30Issue(1)：58-63,6.DOI:10.3969/j.issn.1004-4957.2011.01.012
固相萃取-液相色谱测定复杂基质蔬菜中9种烟碱类残留
Determination of Nicotinoid Residues in Complicated Matrix Vegetables by Solid Phase Extraction and HPLC Method
摘要
Abstract
A simple and sensitive high performance liquid chromatographic(HPLC) method was developed for the simultaneous determination of 9 nieotinoid insecticide residues, including dinotefuran,nitenpyram, flonicamid, thiamethoxam, imidacloprid, clothianidin, imidaclothiz, acetamiprid and thiacloprid, in sulphur-containing vegetables samples. The extract efficiencies of several solvent and the cleanup capacities of different SPE cartridges were compared. The vegetables were extracted with acetonitrile and cleaned up on an Envi - Carb/PSA cartridge. The nicotinoid insecticides were eluted with acetonitrile and detected with UV detector. The calibration curves showed good linearities in the range of 0.02 - 1.0 mg/L with correlative coefficient more than 0.999. The limits of quantitation (LOQ) were 0. 02 mg/kg for all 9 pesticide except for thiacloprid with LOQ of 0.05 rog/kg. The recoveries of 9 pesticides from vegetable sample spiked at three concentration levels of 0.02 mg/kg,0.05 rog/kg and 0.5 mg/kg ranged from 67％ to 110％ with RSDs less than 18.6％. The established method was simple, sensitive and accurate, and was suitable for the routine detection of nicotinoid pesticides residues in vegetables.关键词
烟碱类残留/杀虫剂/高效液相色谱/固相萃取/蔬菜Key words
nicotinoid residue/ insecticide/ HPLC/ SPE/ vegetable分类
化学化工引用本文复制引用
侯如燕,卞红正,赵秀霞,胡祎芳,苏婷,王孝辉,宛晓春..固相萃取-液相色谱测定复杂基质蔬菜中9种烟碱类残留[J].分析测试学报,2011,30(1):58-63,6.基金项目
农业部现代农业产业技术体系(农科教发[2008]10号) （农科教发[2008]10号）
农业行业标准制定和修改项目(农财发[2010]49号) （农财发[2010]49号）
