分析测试学报2011，Vol.30Issue(1)：85-90,6.DOI:10.3969/j.issn.1004-4957.2011.01.018
大孔吸附树脂分离纯化金银花绿原酸研究
Study on Separation and Purification of Chlorogenic Acid from Honeysuckle Flower by Macroporous Resin
摘要
Abstract
The tests of single factor, orthogonal and verification to separate and purify chlorogenic acid from honeysuckle crude extracts by NKA-2, D1400, polyamide, HP2MGL, ADS-21, D101 and AB-8 seven macroporous resin were carried out in sequence, and the best processing parameters were optimized based on the adsorption and desorption results. The results showed that NKA-2 was the best resin, whose static saturated adsorption capacity was 212. 17 mg/g, and desorption rate by 90％ ethanol reached 81.4％. The best processing parameters for separating and purifying chlorogenic acid from honeysuckle crude extracts by NKA-2 resin were as follows: sample concentration: 4 g/L, sample volume: 3.5 BV, sample flow rate: 3 BV/h, elution ethanol concentration: 80％ , elution volume: 3 BV, and elution flow rate: 1 BV/h. Under the optimal conditions, the yield rate of chlorogenic acid reached 85.79％ , and its purity was 45.50％. When using sample concentration of 3 g/L, sample volume of 4. 5 BV, sample flow rate of 2 BV/h, elution ethanol concentration of 80％ , elution volume of 3 BV and elution flow rate of 3 BV/h, the purity of chlorogenic acid reached 53.07％ , and its yield was 73.33％. Comparing with the current separating and purifying process of chlorogenic acid from honeysuckle crude extracts, the optimized process has better practical and reference value.关键词
金银花/绿原酸/大孔吸附树脂/静态吸附/动态吸附/分离纯化Key words
honeysuckle flower/ chlorogenic acid/ macroporous resin/ static adsorption/ dynamic adsorption/ separation and purification分类
化学化工引用本文复制引用
龚志华,任国谱,舒青孝,肖文军..大孔吸附树脂分离纯化金银花绿原酸研究[J].分析测试学报,2011,30(1):85-90,6.基金项目
国家APEC基金资助项目(200847) （200847）
中国博士后基金资助项目(20070410985) （20070410985）
国家"十二五"支撑计划资助项目(2011BAD10B00) （2011BAD10B00）
