分析测试学报2011，Vol.30Issue(1)：68-71,4.DOI:10.3969/j.issn.1004-4957.2011.01.014
磺氯酚N-Cu(Ⅱ)-HSA-OP体系对尿蛋白的测定
Determination of Urine Protein by Sulfur Chlorophenol N - Cu(Ⅱ) - HSA - OP System
摘要
Abstract
In pH 3.0 Britton - Robinson buffer, sulfur chlorophenol N bonded with Cu ( Ⅱ ) to form a blue complex, and the complex reacted with protein to form a dark blue multi-complex at room tem perature which presents a maximum absorption at 724 nm and a red shift of 174 nm comparing with that of sulfur chlorophenol N. A new method for the determination of proteins was established based on the reaction of multi-compound substance. The spectral characterisitics of the system and its influ encing factors were investigated. Under the optimal conditions, the apparent molar absorptivity and linear range for HSA were 3.06 × 10s L · mol-1 · cm-1 and 20 - 120 nag · L-1 , respectively. With the presence of polyoxyethylene octyphenyl ether ( OP), the sensitivity of the method could be in creased by 31％. The mehtod was applied in the determination of urine protein with satisfactory re sults. And the sensitivity of the method is 16 times higher than that of the Biuret method.关键词
光度法/磺氯酚N-Cu(Ⅱ)络合物/尿蛋白Key words
spectrophotometry/ sulfur ehlorophenol N -Cu( Ⅱ ) complex/ urine protein分类
化学化工引用本文复制引用
胡秋娈,冯爱青,赵淑珍,刘尚才..磺氯酚N-Cu(Ⅱ)-HSA-OP体系对尿蛋白的测定[J].分析测试学报,2011,30(1):68-71,4.基金项目
河南省科技厅科技攻关重点项目(102102110154) （102102110154）
河南省教育厅自然科学基金资助项目(200510482009) （200510482009）
