分析测试学报2011，Vol.30Issue(2)：167-170,175,5.DOI:10.3969/j.issn.1004-4957.2011.02.009
聚丙烯酸的凝胶渗透色谱分离及相对分子质量测定
Separation of Polyacrylic Acid and Determination of Its Relative Molecular Mass by Gel Permeation Chromatography
摘要
Abstract
Using Sepax Nanofilm SEC-150 chromatographic column and self-made narrow distribution polyacrylic acid (PAA) standard sample, effects of different inorganic salts and their ionic strength as well as pH value on the separation of PAA were investigated. The results indicated that the different relative molecular masses of PAA with mass concentration of 0. 5 - 2. 0 g/L could be separated by using ionic strength of 0. 10 mol/L and pH 6. 9 phosphorous buffer as mobile phase, with a flow rate of 1.0 mL/min, column temperature of 25 ℃ and sample volume of 50 μL. Under the optimal chromatographic conditions, the relative molecular mass and its distribution of PAA sample were measured accurately by the asymptotic calibration method, and the difference between result of viscometric and GPC methods was less than 10％.关键词
聚丙烯酸/相对分子质量/凝胶渗透色谱/非体积排除效应Key words
polyacrylic acid/ molecular weight/ gel permeation chromatography ( GPC )/ non-size exclusion effect分类
化学化工引用本文复制引用
杜小弟,张安富,雷家珩,巫辉..聚丙烯酸的凝胶渗透色谱分离及相对分子质量测定[J].分析测试学报,2011,30(2):167-170,175,5.基金项目
交通部西部交通建设科技项目(2007319811130) （2007319811130）
