军事医学2011，Vol.35Issue(1)：63-66,4.
核因子κB降低小鼠胚胎成纤维细胞中Lmp2基因转录
NF-κB decreases the level of Lmp2 genes in murine embryo fibroblasts
崔艳 1陈婷 1秦雪梅 1孙梦梅 2李平 1曹诚2
作者信息
- 1. 军事医学科学院生物工程研究所,北京,100850
- 2. 安徽大学生命科学学院,合肥,230039
- 折叠
摘要
Abstract
Objective To investigate the possibility of nuclear factor-kB(NF-kB) involved in the regulation of Lmp2 in murine embryo fibroblasts(MEF). Methods The endogenous NF-kB activity is detected using a NF-kB luciferase reporter (pNFkB-Luc) in the MEFs and c-abl -/-/arg-/- MEFs (c-abl-/-/arg-/- -double knockout, DKO). The dual-luciferase reporter assay system was used to evaluate the effect of NF-kB on the transcription of Lmp2, and the expression level of Lmp2 was detected by quantitative PCR and Western blotting. Results The NF-kB activity was upregulated in c-abl/arg deficient fibroblasts. The promoter activity of Lmp2 was decreased in DKO cells, while increased when NF-kB box was mutated. Then, the mRNA and protein level of Lmp2 were downregulated in DKO cells. Conclusion NF-kB is a negative regulator of the Lmp2 genes in MEF.关键词
NF-κB/小鼠胚胎成纤维细胞/Lmp2/转录启动子分类
生物科学引用本文复制引用
崔艳,陈婷,秦雪梅,孙梦梅,李平,曹诚..核因子κB降低小鼠胚胎成纤维细胞中Lmp2基因转录[J].军事医学,2011,35(1):63-66,4.
