军事医学2011，Vol.35Issue(1)：48-53,6.
细菌蛋白质组双向电泳中"串联珠"现象初探
Protein spot train in 2-DE gels for bacterial proteome:a primary research
摘要
Abstract
Objective To find the possible reason for making spot train in 2-DE gels of bacterial proteome. Methods The 2-DE gels with different sample preparation methods were compared to discover the key factor for making spot train.The spots forming a train were cut off form the gels and digested by trypsin, then analyzed by MALDI-TOF/TOF. Results Spot train would appear when the protein samples were boiled in lysis buffer containing 0. 5％ SDS. The spots on one train were identified to be the same protein by MALDI-TOF/TOF. No significant shift of the mass peaks was observed in the PMF spectra of these protein spots. Conclusion SDS would lead to a certain modification or tight binding to proteins when boiled, especially those acidic proteins with large relative molecular mass.关键词
双向电泳/基质辅助激光解吸电离飞行时间质谱/串联珠/十二烷硫酸钠分类
生物科学引用本文复制引用
胡威,朱力,商娜,王雪芳,徐慧杰,冯尔玲,魏华,王恒樑..细菌蛋白质组双向电泳中"串联珠"现象初探[J].军事医学,2011,35(1):48-53,6.基金项目
国家自然科学基金项目(30700035) （30700035）
国家973计划项目 (2005CB522904) （2005CB522904）
