云南农业大学学报2011，Vol.26Issue(1)：26-29,4.DOI:10.3969/j.issn.1004-390X(n).2011.01.005
CTAB法用于染病烟草植株中烟草丛顶病毒RNA的提取
Application of CTAB Method for Tobacco Bushy Top Virus RNA Extraction from Infected Tobacco Plants
摘要
Abstract
CTAB method is one of the conventional pathogenic total nucleic acids extraction ways from diseased plants, which can then be used for pathogen detection in PCR-based techniques.This method has been widely used in total nucleic acids extraction and pathogen detection from the plants infected by DNA virus, phytoplasma, bacterium and fungus.In this study, the CTAB method was tried to extract the total nucleic acids from infected tobacco plants by a RNA virus of Tobacco bushy top virus (TBTV) , and compared with Trizol method, a routine RNA virus nucleic acids extraction method, for TBTV RT-PCR detection.The RT-PCR detection results showed that TBTV expected cDNA band could be amplified from the RNA templates extracted from both two methods.The extracts include both DNA and RNA when CTAB is used to extract the total nucleic acids from the co-infected plants, and thus are beneficial to detect both DNA and RNA pathogens.The chemicals used in CTAB method are much cheaper and low toxic than Trizol reagent.Additionally, the total nucleic acids extracted with CTAB method are high quantity and have long-term storage compared with Trizol method.Therefore, CTAB method is a more efficient , simple and cheap method for RNA extraction from TBTV infected tobacco plants.关键词
CTAB法/Trizol法/核酸提取/烟草丛顶病毒分类
农业科技引用本文复制引用
左瑞娟,周雨泫,李丽娟,董鹏,陈海如,李凡..CTAB法用于染病烟草植株中烟草丛顶病毒RNA的提取[J].云南农业大学学报,2011,26(1):26-29,4.基金项目
国家自然科学基金项目(30660101). （30660101）
