云南农业大学学报2011，Vol.26Issue(1)：34-40,7.DOI:10.3969/j.issn.1004-390X(n).2011.01.007
水牛β-LG基因3,4外显子遗传特征分析
Genetic Characteristics of the Exon 3 and 4 of β-LG in Buffalo
摘要
Abstract
In this study, exon 3 and exon 4 plus their flanking segments of β - LG were amplified and sequenced in buffalo firstly.The polymorphisms of exon 4 of β - LG in 224 buffaloes belonging to 10 populations were investigated by means of PCR - RFLP and PCR - SSCP.Then we selected randomly 69 individuals from the 224 buffaloes to detect their polymorphisms of exon 3 of β - LG by PCR - SSCP and sequencing.The results showed that exon 3 and exon 4 of β - LG in both river and swamp buffalo were 74 bp and 111 bp long, respectively.The sequences of exon 3 and exon 4 of β - LG had 100％ identity and monomorphism were observed for the buffalo populations studied.Compared with homologous sequence of bovine species, the sequences of exon 3 and exon 4 of β - LG are conservative, and the protein sequences in buffalo is the same with those coding by B allele in Bos taurus.There was no difference in the sequences of exon 3 and exon 4 of β - LG between both kind of buffalo, but they have significant difference in milking performance, this indicates that the exon 3 and exon 4 of β - LG haveno direct effect on milk yield in buffalo.关键词
河流型水牛/沼泽型水牛/β-LG基因/PCR-RFLP/PCR-SSCP分类
农业科技引用本文复制引用
祁宏,李卫真,袁峰,李大林,刘丽仙,钱坤,苗永旺..水牛β-LG基因3,4外显子遗传特征分析[J].云南农业大学学报,2011,26(1):34-40,7.基金项目
云南省应用基础研究重点项目(2007C0003Z) （2007C0003Z）
国家自然科学基金项目(30660024) （30660024）
云南省应用基础研究计划面上项目(2006C0034M,2007C057M,2010ZC243) （2006C0034M,2007C057M,2010ZC243）
国家高技术研究发展计划("863"计划)项目(2008AA101001) （"863"计划）
云南省教育厅科学研究基金项目(09C0197,2010Y344). （09C0197,2010Y344）
