四川医学2011，Vol.32Issue(2)：145-148,4.
大鼠入肝血流与半肝血流阻断下肝脏损伤的随机对照实验
Comparison of total hepatic and hemihepatic blood inflow occlusion in rat: a prospective randomized trial
摘要
Abstract
Objective To compare the survival time and rate 、pathological changes、the ultrastructure of liver cells between the total hepatic blood inflow occlusion group( TH) and the hemihepatic blood inflow occlusion group( HH) in a rat model. Methods Sixty male SD rats subjected to hepatic blood inflow occlusion were randomly assigned to TH or HH. The two ischemic-reperfusion groups included three different ischemia period : 30 minutes , 45 minutes and 60 minutes with 30 minutes reperfusion. After reperfusion , left liver resection was performed for pathologic determination. Liver injury was evaluated using rats survival rate and time.pathologic changes by hematoxylin and eosin( H＆E) staining.ultrastructural changes by transmission electron microscope( TEM) . Results Rats survival rate of postoperative day 7 in HH group was significantly higher than in TH group. HH was associated with better animal survival time compared with TH ( P = 0. 00 , P ＜ 0. 05 ) . Statisticaldifference was presented between TH and HH groups at different ischemia-reperfusion period with P value being 0. 055 ,0. 000 ,0. 000 , respectively. On the basis of statistical analysis, there were no differences on the examination of H&E-stained tissue slides between HH and TH group( P = 0. 484 ) . However,the more time the occlusion was performed, the more injury of hepatocyte was present ( P = 0. 002 ) . The characteristics under TEM included mitochondria seelling , margination of chromatin , endothelial cell swelling , dilated sinusoidal space , steatosis , apoptosis body. Conclusion The survival time and rate in HH group within 60 minutes in rats is superior to TH group. There are no pathological changes between two groups ; Within 60 minutes of blood inflow occlusion, the ultrastructural changes of liver cells are reversible.关键词
入肝血流阻断/半肝血流阻断/病理学/超微结构分类
医药卫生引用本文复制引用
杨健,吴国长,文天夫..大鼠入肝血流与半肝血流阻断下肝脏损伤的随机对照实验[J].四川医学,2011,32(2):145-148,4.基金项目
四川省卫生厅科研项目(编号:0040205301185) （编号:0040205301185）
