现代检验医学杂志2011，Vol.26Issue(1)：59-62,4.DOI:10.3969/j.issn.1671-7414.2011.01.022
利用溶血指数评价溶血对四项生化检测指标的干扰
Hemolytic Index for Assessing the Interference of Hemolysis Against Bio-Indicators
陈建芸 1石玉玲 1李林海 1胡志恒 1李晓1
作者信息
- 1. 广州军区广州总医院检验科,广州,510010
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摘要
Abstract
Objective To investigate the interference of hemolysis with four biochemical items through hemolytic index ,and to quantify the influence degree of hemolysis index,and to proof the ability of antiinterference of kit to avoid misdiagnosis and mistreatment. Methods According to the standard procedure files EP7-A2 established in the clinical laboratory standardization commission (CLSI) ,made a project to detect the relationship between hemoglobin concentrations and hemolysis index.and the hemolysis influence to the glucose (GLU) .blood urea nitrogen (BUN) , aspartate aminotransferase (AST) and total cholesterol(TC). Collected the fresh mixture serum from 10 cases within the reference,5 cases of low value and 5 cases of high value,made 5 different concentrations hemolysis model as experiment group and paired with hemolysisless model as control group. Hemoglobin concentrations were detected in the two groups with Sysmex-XS-800i,and the four items and hemolysis index of the two groups and the original mixture serum were detected for three times in Roche Cobas C501 automatic biochemistry analyzer. Made a disturbing curve for the X axis of the hemolysis index of mixed serum and Y axis of the corresponding detection program changes (interference). And refered to the statistical methods of EP7-A2,work out the disturbing concentration corresponding the maximum allowable deviation. Results The statistical results showed that U value of BUN and TC was 18. 38,4. 05(P＜O. 05) ;U value of was 0. 65(P＞O. 05). If hemolysis index H ＜26,U= 0. 42 , P＞ 0. 05 and if H＞49,U= 41. 91, P＜ 0. 05. The interference dose response curve was made through the statistics regression analysis. The interference dose regression equation of GLU,BUN,AST and TC were Y= 0. 000 1X0. 024 8.Y= 0. 001 9X- 0. 223 7.Y= 0. 065 4X- 0. 275 9 and Y= 0. 000 SX+O. 000 4 respectively. According to the inter ference dose response curve ,get that hemolysis didn't produced obviously disturb to the results of GLU,The ability of anti-hemolysis interference of BUN ,AST and TC be equal to instruction of kit. Conclusion The hemolysis index could be used to judge the sample hemolysis or not ,degree of hemolysis and extent of interference,and provide objective evidencefor clinic to avoid misdiagnosis and mistreatment.关键词
溶血指数/溶血/干扰/生化检验分类
医药卫生引用本文复制引用
陈建芸,石玉玲,李林海,胡志恒,李晓..利用溶血指数评价溶血对四项生化检测指标的干扰[J].现代检验医学杂志,2011,26(1):59-62,4.
