山东医药2011，Vol.51Issue(6)：10-11,2.
雷公藤内酯醇对耐顺铂人卵巢癌细胞株COC1/DDP增殖和凋亡的影响
Effects of triptolide on prolifration and apoptosis of cisplatin-resistance human ovarian cancer line COC1/DDP
摘要
Abstract
Objective To study the effects of triptolide (TP) on prolifration and apoptosis of cisplatin-resistance human ovarian cancer line COC1/DDP. Methods MTT assay was used to analyse growth inhibition effect of DDP(0.5μg/ml) and medium concentration TP ( 10 ng/ml) on COC1/DDP cells. 24 hours after the treatment of TP, the COC1/DDP ultrastructure by transmission electron microscopesh was detected. The apoptosis fracture zone investigated with DNA electrophoresis. Results Medium concertration TP could evidently inhibit the growth of COC1/DDP cells,and inhibitory effect on COC1/DDP in a time-dependent manner(P ＜0.05). 24 hours after the treatment of TP with DDP, microvillus on the surface of COC1/DDP cells disappeared, cell-nucleus was condensed, and chromosomal was agglutinated into crescent, apoptosis body had formed. DNA trapezoid strips could be seen with agarose gelelectrophoresis. Conclusions 10 ng/ml TP can inhibit the growth and promote the apoptosis of COC1/DDP.关键词
卵巢癌/雷公藤内酯醇/细胞增殖/细胞凋亡分类
医药卫生引用本文复制引用
胡辉,谭布珍,袁铿,胡银英,黄艳琴..雷公藤内酯醇对耐顺铂人卵巢癌细胞株COC1/DDP增殖和凋亡的影响[J].山东医药,2011,51(6):10-11,2.基金项目
江西省科技支撑计划资助项目(GJJ08074). （GJJ08074）
