烟草科技Issue(1)：70-73,4.
利用Real-time RT-PCR法检测抗病毒活性物质对CMV复制的影响
Real-time Reverse Transcription PCR for Assessment of CMV Multiplication Affected by Antivirus Substance
摘要
Abstract
To accurately test the content of virus in tobacco after treated by antivirus substance, a real - time PCR assay system was established.A bacteria strain antagonistic to the cucumber mosaic virus (CMV) , B6, was isolated from a field of tobacco.The isolated and purified antivirus substance is referred to as B6p, its relative molecular weight is 40.6 kDa.The virus multiplication characteristics in B6p treated host were analyzed with a round leaf method, and the results showed that B6p inhibited the multiplication of virus in the host, and the multiplication action weakened with the increase of antivirus substance concentration.关键词
real-time RT-PCR/病毒/活性物质/CMV分类
农业科技引用本文复制引用
王芳,高正良,周本国,雷艳丽,许大风,李英..利用Real-time RT-PCR法检测抗病毒活性物质对CMV复制的影响[J].烟草科技,2011,(1):70-73,4.基金项目
安徽省烟草专卖局资助项目"皖北烟草脉斑病的发生规律及防治技术研究与应用(AHKJ2008-02)". （AHKJ2008-02）
