现代检验医学杂志2011，Vol.26Issue(1)：22-27,6.DOI:10.3969/j.issn.1671-7414.2011.01.009
红细胞参数区分小红细胞人群中珠蛋白生成障碍性贫血基因携带者与铁缺乏症的诊断价值
Diagnostic Evaluation of RBC Parameters in Differentiation Thalassemia Carriers from Iron Deficiency in Microcytosis Population
摘要
Abstract
Objective To evaluated the diagnostic value of red blood cell (RBC) count and other five discrimination indices or formula in differentiation thalassemia carriers from iron deficiency in microcytosis population(MCV＜82fl). Methonds 1 673 'healthy people'that took physical examination were included in current study. All people received 7 RBC parameter analysis sera iron . serum iron (SI) and hemoglobin electrophoresis. ID was diagnosis by SI (Male : SI＜1O μmol/l_ , Female :SI＜9. O μmol/L). Furthermore , 6 a and 17 β-thalassemia gene analysis was conducted in the peoples whose mean cell volume (MCV) lower than 82 fl. The diagnostic value of RBC count and other index or mathematical formulas for distinguishing thalassaemia carrier from iron deficiency were evaluated by the receiver operative characteristic cure (ROC). In addi tion,cut-off value,sensitivity,specificity and Youden's index were calculated from the ROC. Results 164 cases of micro cytosis (MCV＜82 fl) were found in 1673 natural population. The ratio of α-thalassemia , β-thalassemia and ID were 47 ％ ,15. 2％ and 35. 4％ respectively. Based on the ROC curve ,we could found that the diagnostic value of RBC count was more outstanding than others in distinguishing thalassaemia carrier from ID. The best cutoff point of RBC count for predicting the presence of the athalassemia or β-thalassemia carriers was 5. 4IX l012/L in men group, giving 82. 5％ sensitivity and specificity of 92. 3％. The Youden index was 74. 8. On the other hand.the best cutoff points of women group was 4. 93×1012/L , the sensitivity, specificity and Youden index were 81. 8％ and 94. 35％ and 76. 1 respectively. Conclusion RBC count was a highly accurate index in predicting α-thalassemia or β-thalassemia carriers in microcytosis population. Because of its high efficacv,stabilization,simplicity,rapiditv,and low cost.it should be recommended for the routine screening ofthalassemia carriers in highly prevalent areas with limited resources.关键词
红细胞数目/珠蛋白生成障碍性贫血/铁缺乏症/受试者工作特征曲线分类
医药卫生引用本文复制引用
林敏,郑磊,蔡贞,杨立业,吴教仁,王前..红细胞参数区分小红细胞人群中珠蛋白生成障碍性贫血基因携带者与铁缺乏症的诊断价值[J].现代检验医学杂志,2011,26(1):22-27,6.基金项目
广东省医学科研基金(A2009781,B2010348). （A2009781,B2010348）
