生命科学研究2011，Vol.15Issue(1)：13-18,6.
斑鳜肌球蛋白轻链2基因cDNA的克隆及其纵向表达分析
Cloning and Longitudinal Expression Analysis of the MCL2 Gene in Siniperca scherzeri
摘要
Abstract
The total RNA was isolated from the white muscle of the Siniperca scherzeri. The full length cDNA of myosin light chain 2 (MCL2) was cloned using RT-PCR technology. The sequence of the MCL2 ORF is 513 bp, and encodes 170 amino acids, which includes four EF-hand structures. The putative protein of the MLC2 shows predicted relative molecular mass of 19 105.61 Da with a theoretical pi of 4.73.Compared with the MLC2 of other six fishes reported, the homology of the deduced amino acids sequences was above 89％. The primary structure of the Ca2+ binding domain was well conserved among the MLC2s of seven fish species. The longitudinal expression analysis by real-time PCR showed that the MLC2 mRNA was expressed along longitudinal axis with some variation among the three muscle positions.关键词
斑鳜/肌球蛋白轻链2/cDNA克隆/纵向表达分类
生物科学引用本文复制引用
赵发兰,陈敦学,农小献,宾石玉,成嘉,蒙涛,张建社,褚武英..斑鳜肌球蛋白轻链2基因cDNA的克隆及其纵向表达分析[J].生命科学研究,2011,15(1):13-18,6.基金项目
国家自然科学基金资助项目(30972263) （30972263）
湖南省教育厅重点资助项目(09A00 （）
10A0108) （）
湖南省高校科技创新团队支持计划资助项目(2008) （2008）
