现代检验医学杂志2011，Vol.26Issue(1)：48-49,53,3.DOI:10.3969/j.issn.1671-7414.2011.01.018
滤膜法万古霉素耐药肠球菌转移方式的研究
Research on Transfer Mode of Vancomycin Resistant Enterococci with Filter Conjugation Test
陈铭 1于艳华 1杨连军 2赵秀英1
作者信息
- 1. 首都医科大学附属北京佑安医院临检中心,北京,100069
- 2. 北京市丰台区南苑医院,北京,100076
- 折叠
摘要
Abstract
Objective To research transfer mode of transposon Tn1546 in VRE in order to provide scientific evidence for clinical infection prevention and epidemic investigation. Methods Conjugation tests were done in VREFm by filter mating methods. According to CLSI's guideline ,Antimicrobial susceptibility tests were performed with disk diffusion method. To detect vancomycin resistant genes in conjugons by PCR. Results VanA genes were transferred successfully from 40 VRE donors to Enterococcus faecalis receptors JH22 by filter mating methods. And part of other resistant genes were also trans ferred simultaneously. VanA genes were detected in all of 40 conjugons and it proved all of 40 VRE were successfully transfered. Conclusion Transposon Tn1546 with VanA genes in VRE could be transfered thoroughly through plasmid conjugation transfer test with interspecies. It causes resistant dissemination in Enterococci. Conjugation test is the important method to research resistant transfer in Enterococcci.关键词
肠球菌/万古霉素/质粒/接合转移分类
医药卫生引用本文复制引用
陈铭,于艳华,杨连军,赵秀英..滤膜法万古霉素耐药肠球菌转移方式的研究[J].现代检验医学杂志,2011,26(1):48-49,53,3.
