现代检验医学杂志2011，Vol.26Issue(1)：152-155,4.DOI:10.3969/j.issn.1671-7414.2011.01.059
梅毒检测方法的临床应用研究
Clinical Applied Research of Syphilis Serological Detection
王珍光 1郭建巍 1马骢 1陈涵 1荣扬1
作者信息
摘要
Abstract
Objective To preliminary assessment the flow sheet of syphilis serological detection by clinical epidemiology and enact practical measurement schema for syphilis serological detection. Methods Perform RPR, ELISA, TPPA and real time PCRsimultaneously with stand operation protocol. Result The sensitivity of combined methods from high to low were (RPR + ELISA + TPPA ) ＞ (RPR + ELISA ) ＞ (RPR + TPPA ) ＞ EL ISA ＞ TPPA ＞ RPR. The specificity of RPR, ELISA and TPPA were over 96 ％. Combined RPR, ELISA and TPPA, the specificity were (RPR + TPPA) ＞ (RPR + ELISA) and (RPR+ELISA+TPPA). As to misdiagnosis rate, (RPR+ELISA)＞ (RPR+TPPA) and (RPR+ELISA+ TPPA). The omission diagnose rate of (RPR+ELISA+TPPA), (RPR+ELISA) and (RPR+TPPA) were 0,0. 99％ and 3.98 ％ respectively. As for precision index, (RPR + ELISA + TPPA) ＞ (RPR + ELISA) ＞ (RPR + TPPA). Positive rate of real time PCR of serological sample was 1 ％. Conclusion Combining RPR,ELISA and TPPA was a more effective and accurate scheme. Serological sample was not suitable for serological diagnosis with real time PCR.关键词
梅毒/血清学检测/流程评价分类
医药卫生引用本文复制引用
王珍光,郭建巍,马骢,陈涵,荣扬..梅毒检测方法的临床应用研究[J].现代检验医学杂志,2011,26(1):152-155,4.
