实用医学杂志2011，Vol.27Issue(3)：369-371,3.DOI:10.3969/j.issn.1006-5725.2011.03.005
冻干重组人三突变型低氧诱导因子-1α腺病毒的制备
Preparation of freeze-dried recombinant adenovirus expressing hypoxia inducible factor-1 alpha of triple mutant
摘要
Abstract
Objective To prepare freeze-dried recomhinant adenovirus expressing hypoxia inducible factor-1 alpha (HIF-lα) of triple mutant (Ad-HIF-lα-564/402/803). Methods Ad-HIF-Iα-564/402/803 were mixed with different stabilizers in an appropriate proportion and then lyophilized. The optimum stabilizer was selected and the product quality was evaluated according to appearance, virus titer, thermostahility, PCR and DNA sequence analvsis. Results PCR and gene sequence suggested the correct construction of freeze-dried recombinant adenovirus. The protective agent containing 10％ trehalose , 0.5％ gelatin, 3％ sorbitol had better protecting effects. After lyophilization, the infectious titer of adenovirus was decreased by 0.33 LgPFU/mL, the titer of lyophilized adenovirus was decreased by 0.8 LgPFU/mL for 3 weeks at 37℃. Conclusions When prepared with proper stabilizer, the freeze-dried recombinant adenovirus expressing HIF-Iα of triple mutant can have a good performance.关键词
缺氧诱导因子1/重组腺病毒/冷冻干燥/保护剂/制备引用本文复制引用
陶宇,杨丽,魏旋,李明琰,陈建威,吴平生..冻干重组人三突变型低氧诱导因子-1α腺病毒的制备[J].实用医学杂志,2011,27(3):369-371,3.基金项目
国家自然科学基金资助项目(编号:30370587) （编号:30370587）
