现代检验医学杂志2011，Vol.26Issue(1)：74-75,78,3.DOI:10.3969/j.issn.1671-7414.2011.01.027
紫癜性肾炎患儿外周血CD4+CD25+调节性T细胞的临床意义
Significance of CD4+CD25+ Regulation T Cells in Children with Nephritis of Schonlein-Henoch Purpura
孟凡茹 1庞晓璐 1曹青 1周艳1
作者信息
- 1. 河北医科大学第二医院检验科,石家庄,050000
- 折叠
摘要
Abstract
Objective To investigate the amount of CD4+CD25+ regulation T cells ,the level of related cytokine and the expression of Foxp3 in peripheral blood in children with HSPN ,and explore the correlation of CD4+CD25+ regulation T cells with the pathogenesis of HSPN. Methods 40 patients with HSPN and 20 age matched healthy children were detected.Flow cytometric analysis (FCM) was performed to detect the percentage of CD4+CD25+ regulation T cells subpopulation.Enzyme-linked immunosorbent assay(ELISA) was used to measure the levels of related cytokines (IL-10 and TGF-β) and real-time PCR were used to analyse Foxp3 expression. Results Compared with control group,CD4+CD25+ regulation T cells were significantly lower in peripheral blood in HSPN children ,and the expression of Foxp3 mRNA were decreased obviously also (P＜0. 05). IL-10 level was markedly lower and TGF -β1 level was significantly higher in HSPN children. The percentage of CD4+CD25+ regulation T cells correlated negatively with renal pathologic grade in HSPN (r=-0. 966 ,P＜ 0. 01). Conclusion Cellular immunological function disorder is observed in HSPN children. The decrease or afunction of CD4+CD25+ regulation T cells will participate in and promote the development of HSPN of children. And its percentage correlate with renal pathologic grade in HSPN.关键词
紫癜性肾炎/CD4+CD25+调节性T细胞/白细胞介素-10/转化生长因子-β1/Foxp3分类
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孟凡茹,庞晓璐,曹青,周艳..紫癜性肾炎患儿外周血CD4+CD25+调节性T细胞的临床意义[J].现代检验医学杂志,2011,26(1):74-75,78,3.
