中国组织工程研究与临床康复2011，Vol.15Issue(1)：41-45,5.DOI:10.3969/j.issn.1673-8225.2011.01.009
骨髓间充质干细胞对免疫介导再生障碍性贫血的治疗
Bone marrow mesenchymal stem cells for the treatment of immune induced aplastic anemia
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have immunomodulatory effects on lymphocytes and other immune-associated cells. Supportive effects of BMSCs on hematopoietic cells had been certified. Occurrence and development of aplastic anemia are closely associated with T cell hyperfunction-induced hematopoietic stem/progenitor cells. OBJECTIVE: To compare the therapeutic effects of BMSCs and whole bone marrow cells in immune-induced aplastic anemia mice. METHODS: Immune-induced aplastic anemia BALB/C mouse models were established. The BALB/C mice were randomly assigned to normal, control, BMSC injection and whole bone marrow cell groups. Therapeutic effects of BMSCs and whole bone marrow cells, volume of hemopoietic tissues, adipocytes and blood sinus structure were observed. RESULTS AND CONCLUSION: ①On day 20, all mice without treatment (8/8) and 4 of 8 mice which received BMSCs died, while 7 of 8 mice were infused with bone marrow cells and kept live. ②No significant difference in change of weight, blood cells, haematopoiesis was detected between BMSCs or bone marrow cells. ③In spleen cells of improved mice with BMSCs or bone marrow cells, the percentages of CD4+T cells, CD8+T cells and the ratio of CD4+/CD8+ were similar. In peripheral blood, the percentages of CD4+T cells and CD8+T cells were lower in BMSCs group than in normal group. The level of CD8+T cells was lowest in mice with bone marrow cells. These results indicate that BMSCs improve aplastic anemia symptom and recover hematopoiesis. The bone marrow cells were more effective than BMSCs in the therapeutical effect, but no significant difference was determined.关键词
再生障碍性贫血/骨髓间充质干细胞/BALB/C鼠/移植/免疫分类
医药卫生引用本文复制引用
黄颖,郑翠玲,杨少光,韩忠朝..骨髓间充质干细胞对免疫介导再生障碍性贫血的治疗[J].中国组织工程研究与临床康复,2011,15(1):41-45,5.基金项目
云南省科技计划项目(2008ZC134M),课题名称:脐带间充质干细胞对再生障碍性贫血患者T细胞的免疫调节作用机制研究. （2008ZC134M）
