实用医学杂志2011，Vol.27Issue(4)：565-568,4.DOI:10.3969/j.issn.1006-5725.2011.04.006
外源性PDGF-DD蛋白对肝癌细胞株BEL-7402的体外作用
The effect of exogenous PDGF-DD protein on human hepatic carcinoma cell line BEL-7402 in vitro
摘要
Abstract
Objective To investigate the effect of exogenous PDGF-DD protein on proliferation and metastasis related gene (VEGF and CXCR4) of PDGF-D and β-PDCJFR positive expressed human hepatic carcinoma cell line BEL-7402. Methods BEL-7402 cells were exposed to different concentrations of PDCJF-DD protein (0 , 5 ,10, 20 , 50 , 100 pg/mL) for 48 h , and cell proliferation was detected by MTT assay. The flow cytometer (FCM) was used to analvze the distribution of cells cvcle. The mRNA expressions of VEGF and CXCR4 were detected by RTPCR. Results Exogenous PDGF-DD protein promoted proliferation of cells in a dose-dependent manner from 10 pg/mL to 100 pg/mL (P＜0.05). Incubation of tumor cells with PDGF-DD markedly decreased the percentage of the G0/G1 -phase and increased that of the S-phase. Compared with control group (0 pg/mL) , mRNA expression of VEGF and CXCR4 (from 10 pg/mL to 100 pg/mL) were increased (P＜0.05 ). Conclusions Exogenous PDGF-D signaling may play a crucial role in the carcinogenesis , development and metastasis of hepatocellular carcinoma. It can be used as a prognostic factor and a potential target for therapy in hepatocellular carcinoma.关键词
肝肿瘤/PDGF-D/BEL-7402/VEGF/CXCR4引用本文复制引用
宋明旭,李莉华,郑纪虎,刘志辉,周希科..外源性PDGF-DD蛋白对肝癌细胞株BEL-7402的体外作用[J].实用医学杂志,2011,27(4):565-568,4.基金项目
无锡市科技局计划项目(编号:CSZ00936) （编号:CSZ00936）
