生命科学研究2011，Vol.15Issue(1)：56-60,5.
巢式甲基化特异性PCR检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子区甲基化
Detection of Promoter Hypermethylation of RASSF1A and CDH13 Gene by Nested Methylation Specific Polymerase Chain Reaction in Hepatocellular Carcinoma Patients
摘要
Abstract
To evaluate the methylation status of the promoter region of RASSF1A and CDH13 gene using serum samples from hepatocellular carcinoma(HCC) patients, nested methylation-specific PCR (nMSP) was used to detect RASSF1A and CDH13 promoter hypermethylation in serum DNA from 32 cases of HCC, while serum samples from 20 healthy individuals were used as controls.The positive rate of promoter hypermethylation in RASSF1A and CDH13 gene were 53.12％ and 31.25％ in the serum of HCC patients.Overall, 68.75％patients with HCC can be detected with promoter hypermethylation in serum DNA.No aberrant promoter methylation was detected in the controls.Clinical data including age, sex, HBV, levels of AFP, clinical stages, invasion and metastasis showed no correlation with aberrant methylation of RASSF1A and CDH13 (P＞0.05).The data showed that it is sensitive for nMSP to detect the aberrant methylation in the promoter of RASSF1A and CDH13 gene in HCC.The detection of RASSF1A and CDH13 gene promoter hypermethylation in the serum of HCC patients can give useful information for the screening, early diagnosis, and prediction of prognosis of HCC.关键词
肝细胞癌/甲基化/RASSF1A基因/CDH13基因/巢式甲基化特异性聚合酶链反应分类
医药卫生引用本文复制引用
龚环宇,刘振国,张浩晔..巢式甲基化特异性PCR检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子区甲基化[J].生命科学研究,2011,15(1):56-60,5.基金项目
湖南省卫生厅科研基金资助项目(BD006090) （BD006090）
