湖北医药学院学报2011，Vol.30Issue(1)：26-28,封4,4.
p27mt对裸鼠肝癌移植瘤组织VEGF表达的影响
Effects of Mutant p27 Gene On VEGF Expression in Nude Mice Transplanted with Hepatocellular Carcinoma
摘要
Abstract
Objective To study the effects of mutant p27 (p27mt) gene on vascular growth of SMMC-7721 ( HCC ) transplated nude mice. Methods The hepatocellular carcinoma model were prepared in nude mice by subcutaneouly transplanting the human hepatocellular carcinoma line SMMC-7721, then adenovirus mediated mutant p27 gene was injected into the transplanted tumor as Ad-p27mt group. The growth of vascular in transplated tumor was observed after 21 d,the expression of VEGF in vascular was detected by immunofluorescence method. Results Compared with Ad-Lac Z group and control group,the tumor vascular were mostly single and thin,and the expression of VEGF was lower in Ad-p27mt group ( P ＜0. 05 ).Conclusion p27mt could inhibit the growth of vascular nourished transplated tumor by decreasing the expression of VEGF.关键词
p27mt/肝癌/VEGF分类
医药卫生引用本文复制引用
何志军,陈先祥,蔡庆和,于洋,杨建业,王家宁..p27mt对裸鼠肝癌移植瘤组织VEGF表达的影响[J].湖北医药学院学报,2011,30(1):26-28,封4,4.基金项目
湖北省自然科学基金项目(2005ABA082) （2005ABA082）
