中国组织工程研究与临床康复2011，Vol.15Issue(2)：302-305,4.DOI:10.3969/j.issn.1673-8225.2011.02.027
脊髓损伤修复相关10号基因编码蛋白与促甲状腺激素释放激素受体2的相互作用
Interaction between spinal cord injury and regeneration related gene No 10 protein and thyrotropin releasing hormone receptor 2
时小燕 1蔺宇2
作者信息
- 1. 河南大学药学院,河南省开封市,475000
- 2. 解放军军事医学科学院基础医学研究所,北京市,100850
- 折叠
摘要
Abstract
BACKGROUND:In primary study, we obtained protein interacting with spinal cord injury and regeneration related gene No 10 (SCIRR10), which involving thyrotropin releasing hormone receptor 2 (TRH R2). However, the interaction between SCIRR10 and TRH R2 has been validated.OBJECTIVE:To explore whether SCIRR10 has effect on TRH R2 and to certify whether TRH R2 is special receptor of SCIRR10.METHODS:Recombinant plasmid expressing SCIRR10-GFP fusion protein and TRH R2-RFP fusion protein were constructed respectively using RT-PCR. The supernatant of these COS7 cells transfected with SCIRR10-GFP was collected and then added into those COS7 cells transfected with TRH R2-RFP, and later cells was cultu red for another 4 hours. Treated cells were washed,fixed with 40 g/L paraformaldehyde, mounted. The results were observed under a laser scanning confocal microscope.RESULTS AND CONCLUSION: RR10-GFP could be seen combined with TRH R2-RFP under fluorescence. No similar results could be found in the control group. The results of this experiment and primary studies demonstrated that, TRHR R2 is the receptor of SCIRR10. However, the interaction protein of SCIRR10 has its special ligand.关键词
脊髓损伤修复相关10/号基因/促甲状腺激素释放激素受体2/脊髓损伤/酵母双杂交/RT-PCR分类
医药卫生引用本文复制引用
时小燕,蔺宇..脊髓损伤修复相关10号基因编码蛋白与促甲状腺激素释放激素受体2的相互作用[J].中国组织工程研究与临床康复,2011,15(2):302-305,4.
