中国组织工程研究与临床康复2011，Vol.15Issue(2)：306-309,4.DOI:10.3969/j.issn.1673-8225.2011.02.028
小鼠囊胚细胞凋亡和半胱氨酸天冬氨酸蛋白酶3表达与维甲酸的诱导作用
Induction effect of retinoic acid on cell apoptosis and Caspase-3 expression in mouse blastocysts
熊彦娥 1张端莲2
作者信息
- 1. 武汉科技大学医学院解剖教研室,湖北省武汉市,430065
- 2. 武汉大学医学院组织胚胎教研室,湖北省武汉市,430071
- 折叠
摘要
Abstract
BACKGROUND:One of the reasons for abnormal development of embryos is the apoptosis induced by retinoic acid, and Caspase-3mediated apoptosis is the main pathway for apoptosis in mouse blastocysts.OBJECTIVE:To observe apoptosis in mouse blastocyst induced by retinoic acid, and to detected expression of Caspase-3 in mouse blastocysts.METHODS:Mouse 3.5 days blastocysts were exposed to doses of 0, 1 and 10 μmol/L retinoic acid for 24 hours. Effects of retinoic acid on mouse blastocysts apoptosis was observed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and the expression of Caspase-3 were detected by immunohistochemical S-P staining.RESULTS AND CONCLUSION:stocysts apoptosis and Caspase-3 expression could be found in all groups, which was significantly higher in the high dosage group than that in the control and low dosage group (P ＜ 0.01). The results suggest that retinoic acid can promote blastocysts apoptosis and Caspase-3 expression in vitro, confirm its cytotoxic effect on mouse embryos.关键词
维甲酸/细胞凋亡/半胱氨酸天冬氨酸蛋白酶3/囊胚/组织构建分类
医药卫生引用本文复制引用
熊彦娥,张端莲..小鼠囊胚细胞凋亡和半胱氨酸天冬氨酸蛋白酶3表达与维甲酸的诱导作用[J].中国组织工程研究与临床康复,2011,15(2):306-309,4.
